Next and Current Generation
DNA Sequencing Technologies
Illumina dye sequencing is method used to determine the series of base pairs in DNA. Researchers at Manteia Predictive Medicine developed this method, then Solexa acquired this method then Illumina. This was based on dye-terminators, this allows them to identify the single bases, and this was also used for sequencing difficult regions in the homopolymers and repetitive sequences.
Helicos single molecule fluorescent sequencing has a platform called Helicos Genetic Analysis System and developed by Helicos Biosciences; it was also the first commercial Next Generation Sequencing. The helicos Genetic Analysis System is based of single molecule fluorescent sequencing, this method identifies the exact sequence of a piece of DNA. This is capable to sequence thousands of nucleotides.
Massively Parallel signature sequencing is used to identify and quantify mRNA transcripts. It’s like Serial Analysis of Gene expression but the biomedical manipulation and sequencing are different. This sequence uses an open-ended platform to analyze the level of gene, to do this it counts the number of individual mRNA that is produced by each gene.
Single molecule real time sequencing is a synthesis technology developed by Pacific Biosciences. This sequence used zero-mode waveguide which was made by Harold G. Craighead and Watt W. Webb at Cornell University. This type of DNA sequencing uses a chip that contain zero-mode waveguide.The zero-mode waveguide have a structure of nanophotonic confinement.
Polony Sequencing is accurate, inexpensive technique that is used to read millions of DNA sequences in parallel. Developed in Harvard Medical School by Dr. George church’s group. This Dna sequence technique can be easily used to set up on epifluorescence microscopy and computer-controlled flow cell system.
Pyrosequencing determines the order of nucleotide in DNA and based “Sequencing by synthesis” principle. In 1996 , Stockholm at Royal Institute of Technology, this was developed by Mostafa Ronaghi and Pal Nyren. “Sequencing by synthesis” this method take a single strand of DNA to sequence then synthesize to its strand enzymatically. Pyrosequencing supposed to detect the activity of DNA polymerase with another chemiluminescent enzyme.
DNA nanoball sequencing is used for for finding the genome sequence of the species; this also a high throughput sequencing technology. To make DNA nanoballs you wolve to use rolling circle replication to amplify small fragment of Genomic DNA. This sequence is a lower cost to other next generation sequencing because this allows larger numbers of nanoballs to be sequenced per run. DNA nanoball sequencing suppose to isolate the DNA that is need to be sequenced, then is analyze the fluorescence data and make a base call.
Illumina Sequencing
Helicos Single Molecule Fluorescent Sequencing
Massively Parallel Signature Sequencing
Single Molecule Real Time Sequencing
Polony Sequencing
Pyrosequencing
DNA Nanoball Sequencing
Next and Current Generation Sequencing
SOLiD sequencing this is also called 2 base encoding, SOLiD is next generation sequencing that it was made by a company made by Applied Biosystem and became available in 2008. SOLiD sequencing can generate thousand of small sequences. The advantages of using SOLiD is that it reads the sequence twice. A disadvantage is that when it miscalls is causes errors on the remaining reads.
SOLiD Sequencing
