Next and Current Generation
DNA Sequencing Technologies
DNA sequencing was first discovered in early 1970s by two researchers named Allan Maxam and Walter Gilbert, which made the first DNA sequencing called Maxam-Gilbert sequencing. The Maxam-Gilbert sequencing method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.
Then on 1977, Frederick Sanger and other colleagues developed a new DNA sequencing method called Sanger sequencing. Sanger sequencing is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.
As DNA sequencing technologies get more researched, they developed many new methods like massively parallel signature sequencing, Polony sequencing, pyrosequencing, Illumina sequencing, SOLiD sequencing, DNA nanoball sequencing, heliscope single single molecule sequencing, Single molecule real time sequencing. Currently there are other DNA sequencing methods that are under development like nanopore DNA sequencing, Tunnelling currents DNA sequencing, sequencing by hybridization, sequencing with mass spectrometry, microfluidic Sanger sequencing, microscopy-based techniques, RNAP sequencing and vitro virus high-throughput sequencing.
The Earlist Methods
Next and Current Generation Sequencing
Sanger Sequencing
Maxam-Gilbert Sequencing
