Next and Current Generation
DNA Sequencing Technologies
Nanopore DNA Sequencing is a method that is currently under development since 1995 and it determines the order in nucleotide occur on DNA strands. What nanopore sequencing supposed to do is a single nucleotide, strands of DNA and other molecules, when they pass through nanopore, it creates characteristic changes in the magnitude through the nanopore. The size of nanopore is a tiny hole of 1 nanometer; there are some nanopores that are more than 10 nanometer. There are two types of nanopores there is alpha hemolysin and MspA.
Alpha hemolysin is a type of nanopore; it cause the lysis of red blood cell. The size of this nanopore in only 10 nanometer long.On top of this nanopore there’s a structure that is like vestibule, and on bottom theres 3 recognition sites, the R1,R2 and R3.There has been recent studies that when R1 and R2 sites enable each base to be monitored is it goes through the nanopore it creates 16 different measurable ionic current values instead of 4; this doubles that sequence that is read per nanopore.
MspA or the mycobacterium smegmatis porin A is a type of biological nanopore that is currently being studied for DNA sequencing. the MspA could used for improving alpha hemolysin because of its structure. The shape of this is shaped as a goblet and the diameter is 1.2 nanometers. The shape of natural MspA is being used for DNA sequencing because the shape doesn’t alloa single stranded DNA translocation. The MspA is being produced by Mycobacteria, this allows hydrophilic nutrients to enter the bacterium
Nanopore DNA Sequencing
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Alpha Hemolysin
MspA
